Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Gibco™ PBS (10X), pH 7.4
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PBS (10X), pH 7.4
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.3 to 7.5 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3100 mOsm/kg |
Tris Buffered Saline Tablets, pH 7.6, Medicago
Supplied as exactly pre-weighed tablets. Medicago Tris Buffered Saline Tablets, pH 7.6 gives 500ml of 1x solution with 0.05M Tris-HCl buffer and 0.15M sodium chloride. Manufactured in ISO 9001 and 13485 certified facilities.
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
Shipping Condition | Room Temperature |
---|---|
Dilution | 10 X |
Content And Storage | Store at room temperature. |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.4 |
Product Line | Ambion™ |
Gibco™ PBS, pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS, pH 7.2
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 280 to 320 mOsm/kg |
Gibco™ ACK Lysing Buffer
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Invitrogen™ TE, pH 8.0, RNase-free
Invitrogen molecular biology grade, TE, pH 8.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use.
Packaging | Bottle |
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Physical Form | Liquid |
pH | 8.0 |
Recommended Storage | Store at room temperature. |
Product Line | Ambion |
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
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Physical Form | Liquid |
pH | 8 |
Grade | Molecular Biology |
Recommended Storage | Store at room temperature. |
Concentration | 1 M |
Product Line | Ambion |
Thermo Scientific™ Dry-blend Buffered Packs
Our quality control ensures that every pack of dry-blend buffers yields consistent solutions without weighing or pH adjustment. Simply empty contents of one foil envelope pack into a beaker, add appropriate solvents, and stir to dissolve.
Form | Powder |
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Product Line | BupH™ |
Gibco™ PBS (10X), pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
PBS (10X), pH 7.2
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.1 to 7.3 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3200 mOsm/kg |
Invitrogen™ Tris (1 M), pH 7.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
---|---|
Physical Form | Liquid |
pH | 7 |
Grade | Molecular Biology |
Recommended Storage | Store at room temperature. |
Concentration | 1 M |
Product Line | Ambion |
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS (10X), no calcium, no magnesium
Dilution | 10 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 6 to 7 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2500 to 2900 mOsm/kg |
Gibco™ DPBS (10X), calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
DPBS (10X), calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Capacity | 500 mL |
pH | 4.3 to 4.7 |
Shelf Life | 24 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2700 to 3200 mOsm/kg |
Gibco™ PB-MAX™ Karyotyping Medium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Fully supplemented medium developed for short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures
Shipping Condition | Dry Ice |
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Content And Storage | Storage conditions: -5 to -20°C. Protect from light. Shipping conditions: Frozen Shelf life: 12 months from date of manufacture |
Form | Liquid |
Color | Red |
Green Features | Sustainable packaging |
For Use With (Application) | Cell Viability; Proliferation & Function; Clinical & Translational Research; Mammalian Cell Culture |
Sterility | Sterile-filtered |
Dilution | 1 X |
Additive | L-Glutamine |
Product Type | Karyotyping Medium |
Sample Type | Lymphocytes |
Species | Human |
Serum Level | Standard Serum |
Product Line | PB-MAX™ |
Gibco™ CTS™ DPBS, without calcium chloride, without magnesium chloride
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Gibco Cell Therapy Systems (CTS) DPBS is the classic Dulbecco's phosphate-buffered saline. This particular formulation lacks calcium, magnesium, and phenol red.
Shipping Condition | Room Temperature |
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Dilution | 1X |
Content And Storage | Store at 15–30°C. |
Additive | No Calcium, No Magnesium |
Form | Liquid |
Product Type | DPBS |
Classification | AOF |
Manufacturing Quality | ISO13485, MDSAP, FDA-registered, 21 CFR820 |
Green Features | Sustainable packaging |
Shelf Life | 36 months from date of manufacture |
For Use With (Application) | Cell and Gene Therapy Research, Development, and Manufacturing |
Sterility | Sterile-filtered |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Gibco™ CTS™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Gibco Cell Therapy Systems DPBS CTS™ is the classical Dulbecco's Phosphate Buffered Saline which contains calcium, magnesium but no phenol red.